Order NO. D191001
产品描述
概述
The 6 X histidine motif is used as a Tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-Tag fused to targeted proteins in transfected or transformed cells.
应用
免疫印迹(WB),免疫荧光(IF),免疫沉淀(IP)
属性
保存缓冲液 |
Ascitic fluid with 0.05% sodium azide and 40% glycerol
|
运输条件 |
2-8°C
|
靶点全称 |
HHHHHH Tag
|
靶点别名 |
6 His epitope Tag
|
靶点研究领域 |
Cell Biology
|
亚型 |
IgG1
|