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Order NO. D191109
小鼠抗EYFP Tag单克隆抗体
英文名 Anti-EYFP Tag mouse monoclonal antibody
别名 Enhanced Yellow florescent protein; eYFP; YFP; Yellow Fluorescent Protein; enhanced Yellow Fluorescent Protein
相关类别 标签抗体 储存 冷冻（-20℃）
宿主 Mouse 反应种属 Fusion proteins containing EYFP
标记物 Unconjugate 克隆类型 mouse monoclonal
抗原 EYFP Tag    
编 号 规格 库存 目录价（￥） 您的价格（￥） 数 量
D191109-0050 50 ul 现货 560 560
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D191109-0100 100 ul 现货 900 900
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属性

Background: Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Applications: WB
Name of antibody: EYFP Tag
Immunogen: Fusion protein of EYFP
Full name: Enhanced Yellow florescent protein
Synonyms: eYFP; YFP; Yellow Fluorescent Protein; enhanced Yellow Fluorescent Protein
WB Predicted band size: 27 kDa
WB Positive control: Recombinant EYFP tag protein
WB Recommended dilution: 5000-20000
相关文档
Sample Application

  • Gel: 12%SDS-PAGE, Lysate: 0.5/0.05 μg, Lane 1-2: Recombinant EYFP tag protein, Primary antibody: D191109(EYFP Tag Antibody) at dilution 1/2400, Secondary antibody: D110087(HRP-conjugated Goat anti mouse IgG) at 1/5000 dilution, Exposure time: 25 seconds