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Order NO. D194671
小鼠抗NDC80单克隆抗体
英文名 Anti-NDC80 mouse monoclonal antibody
别名 NDC80 kinetochore complex component; HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human
克隆类型 mouse monoclonal 抗原 NDC80
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D194671-0050 50 ul 预计5日内发货
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D194671-0100 100 ul 预计5日内发货
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产品描述

概述

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

应用

免疫印迹(WB),免疫荧光(IF),流式细胞术(FCM)

属性

保存缓冲液 -20°C, PBS (PH 7.4) containing 0.05% sodium azide.
运输条件 2-8°C
靶点 Uniprot 登记号 O14777
靶点基因 ID 10403
靶点全称 NDC80 kinetochore complex component
靶点别名 HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
靶点研究领域 Cancer, Epigenetics and Nuclear Signaling
亚型 IgG1
相关文档
Sample Application

  • The image is flow cytometry analysis of Hela cells using D194671(NDC80 antibody) at dilution 1/400 (green) and negative control (red).

  • The image is immunofluorescence of Hela cells using D194671(NDC80 Antibody) at dilution 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Gel: 8%SDS-PAGE, Lysate: 20 μg, Lane 1-5: Hela, HepG2, Jurkat, K562 and A431 cell lysates, Primary antibody: D194671(NDC80 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution, Exposure time: 2 minutes
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