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Order NO. D194861
小鼠抗RHOA单克隆抗体
英文名 Anti-RHOA mouse monoclonal antibody
别名 ras homolog family member A; ARHA; ARH12; RHO12; EDFAOB; RHOH12
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human
克隆类型 mouse monoclonal 抗原 RHOA
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D194861-0050 50 ul 预计5日内发货
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D194861-0100 100 ul 预计5日内发货
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产品描述

概述

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.

应用

免疫印迹(WB), 免疫组织化学(IHC), 免疫荧光(IF), 流式细胞术(FCM)

属性

保存缓冲液 -20°C, PBS (PH 7.4) containing 0.05% sodium azide.
运输条件 2-8°C
靶点 Uniprot 登记号 P61586
靶点基因 ID 387
靶点全称 ras homolog family member A
靶点别名 ARHA; ARH12; RHO12; EDFAOB; RHOH12
靶点研究领域 Signal Transduction
亚型 IgG2b
相关文档
Sample Application

  • The image is flow cytometry analysis of Hela cells using D194861(RHOA antibody) at dilution 1/400 (green) and negative control (red).

  • The image is immunofluorescence of Hela cells using D194861(RHOA Antibody) at dilution 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary

  • The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using D194861(RHOA Antibody) at dilution 1/800. (Original magnification: ×200)

  • The image is immunohistochemistry of paraffin-embedded Human endometrial carcinoma tissue using D194861(RHOA Antibody) at dilution 1/800. (Original magnification: ×200)

  • Gel: 12%SDS-PAGE, Lysate: 20 μg, Lane 1-4: Jurkat, MCF-7, A431 and Hela cell lysates, Primary antibody: D194861(RHOA Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution, Exposure time: 2 minutes
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