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Order NO. D194871
小鼠抗H2AX单克隆抗体
英文名 Anti-H2AX mouse monoclonal antibody
别名 H2A.X variant histone; H2A.X; H2A/X; H2AFX
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human
克隆类型 mouse monoclonal 抗原 H2AX
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D194871-0050 50 ul 预计5日内发货
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D194871-0100 100 ul 预计5日内发货
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产品描述

概述

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

应用

免疫印迹(WB), 免疫组织化学(IHC), 免疫荧光(IF), 流式细胞术(FCM)

属性

保存缓冲液 -20°C, PBS (PH 7.4) containing 0.05% sodium azide.
运输条件 2-8°C
靶点 Uniprot 登记号 P16104
靶点基因 ID 3014
靶点全称 H2A.X variant histone
靶点别名 H2A.X; H2A/X; H2AFX
靶点研究领域 Epigenetics and Nuclear Signaling
亚型 IgG1
相关文档
Sample Application

  • The image is flow cytometry analysis of Hela cells using D194871(H2AX antibody) at dilution 1/400 (green) and negative control (red).

  • The image is immunofluorescence of Hela cells using D194871(H2AX Antibody) at dilution 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D194871(H2AX Antibody) at dilution 1/800. (Original magnification: ×200)

  • The image is immunohistochemistry of paraffin-embedded Human cerebellum tissue tissue using D194871(H2AX Antibody) at dilution 1/800. (Original magnification: ×200)

  • Gel: 12%SDS-PAGE, Lysate: 20 μg, Lane 1-5: Jurkat, Hela, Raji, K562 and HCT 116 cell lysates, Primary antibody: D194871(H2AX Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution, Exposure time: 2 minutes
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