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Order NO. D194945
小鼠抗SIRT1单克隆抗体
英文名 Anti-SIRT1 mouse monoclonal antibody
别名 sirtuin 1; SIR2; SIR2L1; SIR2alpha
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human
克隆类型 mouse monoclonal 抗原 SIRT1
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D194945-0050 50 ul 预计5日内发货
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D194945-0100 100 ul 预计5日内发货
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产品描述

概述

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

应用

免疫印迹(WB), 免疫组织化学(IHC), 免疫荧光(IF), 流式细胞术(FCM)

属性

保存缓冲液 -20°C, Ascitic fluid with 0.05% sodium azide
运输条件 2-8°C
靶点 Uniprot 登记号 Q96EB6
靶点基因 ID 23411
靶点全称 sirtuin 1
靶点别名 SIR2; SIR2L1; SIR2alpha
靶点研究领域 Epigenetics and Nuclear Signaling, Metabolism, Cancer
亚型 IgG1
相关文档
Sample Application

  • The image is flow cytometry analysis of K562 cells using D194945(SIRT1 antibody) at dilution 1/400 (green) and negative control (purple).

  • The image is immunofluorescence of NTERA-2 cells using D194945(SIRT1 Antibody) at dilution 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using D194945(SIRT1 Antibody) at dilution 1/800. (Original magnification: ×200)

  • The image is immunohistochemistry of paraffin-embedded Human kidney cancer tissue using D194945(SIRT1 Antibody) at dilution 1/800. (Original magnification: ×200)

  • Gel: 6%SDS-PAGE, Lysate: 20 μg, Lane 1-5: MCF-7, Jurkat, Hela, HEK293 and A549 cell lysates, Primary antibody: D194945(SIRT1 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution, Exposure time: 2 minutes
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