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Order NO. D194951
小鼠抗PRKAA1单克隆抗体
英文名 Anti-PRKAA1 mouse monoclonal antibody
别名 protein kinase AMP-activated catalytic subunit alpha 1; AMPK; AMPKa1; AMPK alpha 1
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human, Mouse, Rat, Monkey
克隆类型 mouse monoclonal 抗原 PRKAA1
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D194951-0050 50 ul 预计5日内发货
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D194951-0100 100 ul 预计5日内发货
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产品描述

概述

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

应用

免疫印迹(WB), 免疫组织化学(IHC), 免疫荧光(IF), 流式细胞术(FCM)

属性

保存缓冲液 -20°C, Ascitic fluid with 0.05% sodium azide
运输条件 2-8°C
靶点 Uniprot 登记号 Q13131
靶点基因 ID 5562
靶点全称 protein kinase AMP-activated catalytic subunit alpha 1
靶点别名 AMPK; AMPKa1; AMPK alpha 1
靶点研究领域 Signal Transduction, Cancer, Metabolism, Cardiovascular, Neuroscience
亚型 IgG1
相关文档
Sample Application

  • The image is flow cytometry analysis of PC-2 cells using D194951(PRKAA1 antibody) at dilution 1/400 (green) and negative control (purple).

  • The image is immunofluorescence of NTERA-2 cells using D194951(PRKAA1 Antibody) at dilution 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using D194951(PRKAA1 Antibody) at dilution 1/800. (Original magnification: ×200)

  • The image is immunohistochemistry of paraffin-embedded Human brain tissue tissue using D194951(PRKAA1 Antibody) at dilution 1/800. (Original magnification: ×200)

  • Gel: 8%SDS-PAGE, Lysate: 20 μg, Lane 1-9: Jurkat, Hela, HepG2, MCF-7, COS-7, NIH/3T3, K562, HEK293 and PC-12 cell lysates, Primary antibody: D194951(PRKAA1 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution, Exposure time: 2 minutes
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