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Order NO. D195429
小鼠抗PIK3CA (mutated E542Q)单克隆抗体
英文名 Anti-PIK3CA (mutated E542Q) mouse monoclonal antibody
别名 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; MCM; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Recognizes E542Q mutant, but not wild-type Human PIK3CA
标记物 Unconjugate 克隆类型 mouse monoclonal
抗原 PIK3CA    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D195429-0100 100 ul 预计5日内发货 暂不支持线上订购

属性

Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Applications: ELISA, WB, IF
Name of antibody: PIK3CA (mutated E542Q)
Immunogen: A synthetic peptide from the internal region of human PIK3CA which includes the mutation of E542Q
Full name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms: MCM; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha
SwissProt: P42336
ELISA Recommended dilution: 1000-5000
WB Predicted band size: 124 kDa
WB Positive control: Purified His-tagged PIK3CA (mutated E542Q) protein
WB Recommended dilution: 500-1000
IF Recommended dilution: 50-100
相关文档
Sample Application

  • The image is immunofluorescence of HEK293T cells transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA (mutated E542Q) plasmid (right column) using D195429(PIK3CA (mutated E542Q) Antibody) at dilution 1/50.

  • Gel: 6%SDS-PAGE, Lysate: 50 ng, Lane 1-2: Purified His-tagged PIK3CA (mutated E542Q) protein and corresponding wild-type protein, Primary antibody: D195429(PIK3CA (mutated E542Q) Antibody) at dilution 1/500, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 30 seconds
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