Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Applications:
ELISA, WB, IF
Name of antibody:
PIK3CA (mutated E542Q)
Immunogen:
A synthetic peptide from the internal region of human PIK3CA which includes the mutation of E542Q
Purified His-tagged PIK3CA (mutated E542Q) protein
WB Recommended dilution:
500-1000
IF Recommended dilution:
50-100
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Sample Application
The image is immunofluorescence of HEK293T cells transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA (mutated E542Q) plasmid (right column) using D195429(PIK3CA (mutated E542Q) Antibody) at dilution 1/50.
Gel: 6%SDS-PAGE, Lysate: 50 ng, Lane 1-2: Purified His-tagged PIK3CA (mutated E542Q) protein and corresponding wild-type protein, Primary antibody: D195429(PIK3CA (mutated E542Q) Antibody) at dilution 1/500, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 30 seconds