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Order NO. D199693
小鼠抗EIF2S1单克隆抗体
英文名 Anti-EIF2S1 mouse monoclonal antibody
别名 EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
相关类别 一抗 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 Human, Dog, Rat, Monkey
克隆类型 mouse monoclonal 抗原 EIF2S1
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D199693-0100 100 ul 预计5日内发货
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产品描述

概述

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit, and the 52-kD EIF2-gamma subunit. The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

应用

免疫印迹(WB),免疫组织化学(IHC),免疫荧光(IF),流式细胞术(FCM)

属性

保存缓冲液 PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
运输条件 2-8°C
靶点 Uniprot 登记号 P05198
靶点基因 ID 1965
靶点全称 eukaryotic translation initiation factor 2 subunit alpha
靶点别名 EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
靶点研究领域 Epigenetics and Nuclear Signaling
亚型 IgG1
相关文档
Sample Application

  • The image is flow cytometry analysis using D199693(EIF2S1 antibody) at dilution 1/100, staining Jurkat cells(Red), compared to a nonspecific negative control antibody(Blue)

  • The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using D199693(EIF2S1 Antibody) at dilution 1/100

  • The image on the left is immunohistochemistry of paraffin-embedded adenocarcinoma of human colon tissue, on the right is immunohistochemistry of paraffin-embedded adenocarcinoma of human ovary tissue using D199693(EIF2S1 Antibody) at dilution 1/40. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded human pancreas tissue, on the right is immunohistochemistry of paraffin-embedded human prostate tissue using D199693(EIF2S1 Antibody) at dilution 1/40. (Original magnification: ×200)


  • Gel: 10%SDS-PAGE, Lysate: 35μg, Lane 1-9: HepG2, Hela, HT-29, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates, Primary antibody: D199693(EIF2S1 Antibody) at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 15 minutes
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