网 站
反 馈
网 站
指 南
Order NO. D199821
小鼠抗PRKAR1A单克隆抗体
英文名 Anti-PRKAR1A mouse monoclonal antibody
别名 ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; TSE1
相关类别 一抗 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 Human
克隆类型 mouse monoclonal 抗原 PRKAR1A
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D199821-0100 100 ul 预计5日内发货
加入购物车
产品描述

概述

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.

应用

免疫印迹(WB),免疫组织化学(IHC),免疫荧光(IF)

属性

保存缓冲液 PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
运输条件 2-8°C
靶点 Uniprot 登记号 P10644
靶点基因 ID 5573
靶点全称 protein kinase cAMP-dependent type I regulatory subunit alpha
靶点别名 CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
靶点研究领域 Signal Transduction, Cancer, Metabolism
亚型 IgG1
相关文档
Sample Application

  • The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using D199821(PRKAR1A Antibody) at dilution 1/100

  • The image is immunohistochemistry of paraffin-embedded adenocarcinoma of breast tissue using D199821(PRKAR1A Antibody) at dilution 1/40. (Original magnification: ×200)

  • Gel: 10%SDS-PAGE, Lysate: 35μg, Lane 1-9: HepG2, Hela, HT-29, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates, Primary antibody: D199821(PRKAR1A Antibody) at dilution 1/2000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 15 minutes
    我的购物车(0) 结算