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Order NO. D199991
小鼠抗tYFP单克隆抗体
英文名 Anti-tYFP mouse monoclonal antibody
别名 C5DELq14.3; DEL5q14.3
相关类别 标签抗体 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 tYFP
克隆类型 mouse monoclonal 抗原 tYFP
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D199991-0100 100 ul 预计5日内发货
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产品描述

概述

Antenna protein that modulates the color of the bioluminescence emission of the luciferase. In the presence of YFP and only at temperatures below 20 degrees Celsius, luciferase exhibits a bimodal emission spectrum with a new peak at 545 nM (yellow), in addition to the one at 485 nM.

应用

免疫印迹(WB),免疫荧光(IF),流式细胞术(FCM)

属性

保存缓冲液 PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
运输条件 2-8°C
靶点 Uniprot 登记号 P21578
靶点研究领域 Cell Biology
亚型 IgG1
相关文档
Sample Application

  • The image is flow cytometry analysis using D199991(tYFP antibody) at dilution 1/400, staining HEK293T cells transfected with either pCMV6-ENTRY(Red) or empty vector control plasmid (Blue)

  • The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using D199991(tYFP Antibody) at dilution 1/50

  • Gel: 12%SDS-PAGE, Lysate: 5μg, Lane 1-2: HEK293T cells lysates were transfected with the pCMV6-ENTRY control and pCMV6-ENTRY tYFP cDNA for 48 hrs, Primary antibody: D199991(tYFP Antibody) at dilution 1/2000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 2 minutes
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