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Order NO. D220124
兔抗SMAD4多克隆抗体
英文名 Anti-SMAD4 rabbit polyclonal antibody
别名 JIP, DPC4, MADH4, MYHRS
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
克隆类型 rabbit polyclonal 抗原 SMAD4
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D220124-0025 25 ul 现货
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D220124-0100 100 ul 现货
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D220124-0200 200 ul 现货
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产品描述

概述

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

应用

酶联免疫吸附测定(ELISA),免疫印迹(WB),免疫组织化学(IHC)

属性

保存缓冲液 -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
运输条件 2-8°C
靶点 Uniprot 登记号 Q13485
靶点基因 ID 4089
靶点全称 SMAD family member 4
靶点别名 JIP; DPC4; MADH4; MYHRS
靶点研究领域 Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Cardiovascular
亚型 Immunogen-specific rabbit IgG
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using D220124(SMAD4 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-6: A549, 293T, Hela, PC3, Jurkat, Raji cell lysates, Primary antibody: D220124(SMAD4 Antibody) at dilution 1/1100, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 30 seconds
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