兔抗CDC23多克隆抗体,Anti-CDC23 rabbit polyclonal antibody 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. D224484

兔抗CDC23多克隆抗体

英文名	Anti-CDC23 rabbit polyclonal antibody
别名	cell division cycle 23; APC8; CUT23; ANAPC8
相关类别	一抗	储存	冷冻（-20℃）
宿主	Rabbit	反应种属	Human, Mouse
标记物	Unconjugate	克隆类型	rabbit polyclonal
抗原	CDC23
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属性

Background:	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
Applications:	ELISA, WB, IHC
Name of antibody:	CDC23
Immunogen:	Fusion protein of human CDC23
Full name:	cell division cycle 23
Synonyms:	APC8; CUT23; ANAPC8
SwissProt:	Q9UJX2
ELISA Recommended dilution:	5000-10000
IHC positive control:	Human liver cancer
IHC Recommend dilution:	50-200
WB Predicted band size:	69 kDa
WB Positive control:	CACO2, 293T, K562, Hela, HepG2 cell lysates
WB Recommended dilution:	1000-5000

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Sample Application

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D224484(CDC23 Antibody) at dilution 1/75, on the right is treated with fusion protein. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: CACO2, 293T, K562, Hela, HepG2 cell lysates, Primary antibody: D224484(CDC23 Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 10 seconds