网 站
反 馈
网 站
指 南
Order NO. D264590
兔抗LDHB多克隆抗体
英文名 Anti-LDHB rabbit polyclonal antibody
别名 lactate dehydrogenase B; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 LDHB    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D264590-0025 25 ul 现货
加入购物车
D264590-0100 100 ul 现货
加入购物车
D264590-0200 200 ul 现货
加入购物车

属性

Background: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
Applications: ELISA, WB, IHC
Name of antibody: LDHB
Immunogen: Synthetic peptide of human LDHB
Full name: lactate dehydrogenase B
Synonyms: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
SwissProt: P07195
ELISA Recommended dilution: 5000-10000
IHC positive control: Human liver cancer and Human gastric cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 37 kDa
WB Positive control: 293T, Jurkat, A549, Hela, Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D264590(LDHB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using D264590(LDHB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-7: 293T, Jurkat, A549, Hela, Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue lysates, Primary antibody: D264590(LDHB Antibody) at dilution 1/800, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 5 seconds
    我的购物车(0) 结算