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Order NO. D264688
兔抗MRPS30多克隆抗体
英文名 Anti-MRPS30 rabbit polyclonal antibody
别名 mitochondrial ribosomal protein S30; PAP; mL65; PDCD9; S30mt; MRP-S30
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 MRPS30    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D264688-0025 25 ul 现货
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D264688-0100 100 ul 现货
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D264688-0200 200 ul 现货
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属性

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: MRPS30
Immunogen: Synthetic peptide of human MRPS30
Full name: mitochondrial ribosomal protein S30
Synonyms: PAP; mL65; PDCD9; S30mt; MRP-S30
SwissProt: Q9NP92
ELISA Recommended dilution: 5000-10000
IHC positive control: Human lung cancer and Human colorectal cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 50 kDa
WB Positive control: LOVO, HT-29, 293T cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using D264688(MRPS30 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using D264688(MRPS30 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: LOVO, HT-29, 293T cell lysates, Primary antibody: D264688(MRPS30 Antibody) at dilution 1/1200, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 5 seconds
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