The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Applications:
WB
Name of antibody:
CASP3 p17 (cleaved)
Immunogen:
Synthetic peptide of human CASP3
Full name:
caspase 3
Synonyms:
CPP32; SCA-1; CPP32B
SwissProt:
P42574
WB Predicted band size:
32 kDa
WB Positive control:
C6, 3T3, Hela lysates
WB Recommended dilution:
500-2000
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Sample Application
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-3: C6, 3T3, Hela lysates, Primary antibody: D290208(CASP3 p17 (cleaved) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes