This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
Applications:
WB
Name of antibody:
EEF2 (phospho-Thr56/Thr58)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr56/Thr58 of human EEF2
Full name:
eukaryotic translation elongation factor 2
Synonyms:
EF2; EF-2; EEF-2; SCA26
SwissProt:
P13639
WB Predicted band size:
95 kDa
WB Positive control:
Hela, HL-60 lysates
WB Recommended dilution:
500-2000
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Sample Application
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela, HL-60 lysates, Primary antibody: D290437(EEF2 (phospho-Thr56/Thr58) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes