Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Applications:
WB, IF, CHIP
Name of antibody:
Histone H3 (triMethyl-Lys9)
Immunogen:
Synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Full name:
H3.4 histone, cluster member
Synonyms:
H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3
SwissProt:
Q16695
WB Predicted band size:
16 kDa
WB Positive control:
Hela lysates, H3 protein
WB Recommended dilution:
500-2000
IF Positive control:
293T cells
IF Recommended dilution:
50-200
CHIP Recommended dilution:
20-100
相关文档
Sample Application
DiMethyl-Histone H3K9 pAb Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3K9 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the rat ios of the immunoprecipitated DNA to the input.
The image is immunofluorescence of 293T cells using D290635(Histone H3 (triMethyl-Lys9) Antibody) at dilution 1/50 (red). Blue: DAPI
Gel: 12%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela lysates, H3 protein, Primary antibody: D290635(Histone H3 (triMethyl-Lys9) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes