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Order NO. D290635
兔抗Histone H3 (triMethyl-Lys9)单克隆抗体
英文名 Anti-Histone H3 (triMethyl-Lys9) rabbit monoclonal antibody
别名 H3.4 histone, cluster member; H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 Histone H3 (triMethyl-Lys9)    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D290635-0050 50 ul 预计5日内发货
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D290635-0100 100 ul 预计5日内发货
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属性

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Applications: WB, IF, CHIP
Name of antibody: Histone H3 (triMethyl-Lys9)
Immunogen: Synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Full name: H3.4 histone, cluster member
Synonyms: H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3
SwissProt: Q16695
WB Predicted band size: 16 kDa
WB Positive control: Hela lysates, H3 protein
WB Recommended dilution: 500-2000
IF Positive control: 293T cells
IF Recommended dilution: 50-200
CHIP Recommended dilution: 20-100
相关文档
Sample Application

  • DiMethyl-Histone H3K9 pAb Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3K9 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the rat ios of the immunoprecipitated DNA to the input.

  • The image is immunofluorescence of 293T cells using D290635(Histone H3 (triMethyl-Lys9) Antibody) at dilution 1/50 (red). Blue: DAPI

  • Gel: 12%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela lysates, H3 protein, Primary antibody: D290635(Histone H3 (triMethyl-Lys9) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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