This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Applications:
WB, IHC, IF
Name of antibody:
KRT8 (phospho-Ser23)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser23 of human KRT8
Full name:
keratin 8
Synonyms:
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
SwissProt:
P05787
IHC positive control:
Human colon cancer
IHC Recommend dilution:
50-100
WB Predicted band size:
54 kDa
WB Positive control:
K562, Hela lysates
WB Recommended dilution:
500-2000
IF Positive control:
Hela cells
IF Recommended dilution:
50-200
相关文档
Sample Application
The image is immunofluorescence of Hela cells using D290819(KRT8 (phospho-Ser23) Antibody) at dilution 1/50 (green). Blue: DAPI
The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D290819(KRT8 (phospho-Ser23) Antibody) at dilution 1/50. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: K562, Hela lysates, Primary antibody: D290819(KRT8 (phospho-Ser23) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes