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Order NO. D290954
兔抗MRPS18B单克隆抗体
英文名 Anti-MRPS18B rabbit monoclonal antibody
别名 mitochondrial ribosomal protein S18B; mS40; PTD017; S18amt; C6orf14; HSPC183; MRPS18-2; HumanS18a; MRP-S18-2
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 MRPS18B    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D290954-0050 50 ul 预计5日内发货
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D290954-0100 100 ul 预计5日内发货
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属性

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Jul 2008]
Applications: WB, IF
Name of antibody: MRPS18B
Immunogen: Fusion protein of human MRPS18B
Full name: mitochondrial ribosomal protein S18B
Synonyms: mS40; PTD017; S18amt; C6orf14; HSPC183; MRPS18-2; HumanS18a; MRP-S18-2
SwissProt: Q9Y676
WB Predicted band size: 29 kDa
WB Positive control: A549, HL-60, U251, RAW264.7 lysates
WB Recommended dilution: 500-2000
IF Positive control: Hela cells
IF Recommended dilution: 50-200
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Sample Application

  • The image is immunofluorescence of Hela cells using D290954(MRPS18B Antibody) at dilution 1/50 (green). Blue: DAPI

  • Gel: 12%SDS-PAGE, Lysate: 30 μg, Lane 1-4: A549, HL-60, U251, RAW264.7 lysates, Primary antibody: D290954(MRPS18B Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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