Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Applications:
WB
Name of antibody:
NCL (phospho-Thr84)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr84 of human NCL
Full name:
nucleolin
Synonyms:
C23; Nsr1
SwissProt:
P19338
WB Predicted band size:
77 kDa
WB Positive control:
U251MG, HepG2, MCF-7 lysates
WB Recommended dilution:
500-2000
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Sample Application
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-3: U251MG, HepG2, MCF-7 lysates, Primary antibody: D290991(NCL (phospho-Thr84) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes