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Order NO. D291121
兔抗PDHA1单克隆抗体
英文名 Anti-PDHA1 rabbit monoclonal antibody
别名 pyruvate dehydrogenase E1 subunit alpha 1; PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 PDHA1    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D291121-0050 50 ul 现货
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D291121-0100 100 ul 预计5日内发货
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属性

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
Applications: WB, IF
Name of antibody: PDHA1
Immunogen: Synthetic peptide of human PDHA1
Full name: pyruvate dehydrogenase E1 subunit alpha 1
Synonyms: PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A
SwissProt: P08559
WB Predicted band size: 43 kDa
WB Positive control: C6, 3T3 lysates
WB Recommended dilution: 500-2000
IF Positive control: Hela cells
IF Recommended dilution: 50-200
相关文档
Sample Application

  • The image is immunofluorescence of Hela cells using D291121(PDHA1 Antibody) at dilution 1/50 (green). Blue: DAPI

  • Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: C6, 3T3 lysates, Primary antibody: D291121(PDHA1 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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