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Order NO. D291214
兔抗PRKAA1 (phospho-Ser496)单克隆抗体
英文名 Anti-PRKAA1 (phospho-Ser496) rabbit monoclonal antibody
别名 protein kinase AMP-activated catalytic subunit alpha 1; AMPK; AMPKa1; AMPK alpha 1
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 PRKAA1 (phospho-Ser496)    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D291214-0050 50 ul 预计5日内发货
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D291214-0100 100 ul 预计5日内发货
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属性

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Applications: WB, IF
Name of antibody: PRKAA1 (phospho-Ser496)
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser496 of human PRKAA1
Full name: protein kinase AMP-activated catalytic subunit alpha 1
Synonyms: AMPK; AMPKa1; AMPK alpha 1
SwissProt: Q13131
WB Predicted band size: 64 kDa
WB Positive control: Ramos lysate
WB Recommended dilution: 500-2000
IF Positive control: Hela cells
IF Recommended dilution: 50-200
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Sample Application

  • The image is immunofluorescence of Hela cells using D291214(PRKAA1 (phospho-Ser496) Antibody) at dilution 1/50 (green). Blue: DAPI

  • Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane: Ramos lysate, Primary antibody: D291214(PRKAA1 (phospho-Ser496) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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