The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Applications:
WB, IF
Name of antibody:
PRKAA1 (phospho-Ser496)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser496 of human PRKAA1
Full name:
protein kinase AMP-activated catalytic subunit alpha 1
Synonyms:
AMPK; AMPKa1; AMPK alpha 1
SwissProt:
Q13131
WB Predicted band size:
64 kDa
WB Positive control:
Ramos lysate
WB Recommended dilution:
500-2000
IF Positive control:
Hela cells
IF Recommended dilution:
50-200
相关文档
Sample Application
The image is immunofluorescence of Hela cells using D291214(PRKAA1 (phospho-Ser496) Antibody) at dilution 1/50 (green). Blue: DAPI
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane: Ramos lysate, Primary antibody: D291214(PRKAA1 (phospho-Ser496) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes