Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
Applications:
WB, IHC
Name of antibody:
PRKCA (phospho-Thr638)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PRKCA
Full name:
protein kinase C alpha
Synonyms:
AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha
SwissProt:
P17252
IHC positive control:
Human colon cancer
IHC Recommend dilution:
50-100
WB Predicted band size:
77 kDa
WB Positive control:
K562, C6, C6+TPA, 3T3, 3T3+TSA lysates
WB Recommended dilution:
500-2000
相关文档
Sample Application
The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D291222(PRKCA (phospho-Thr638) Antibody) at dilution 1/50. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-5: K562, C6, C6+TPA, 3T3, 3T3+TSA lysates, Primary antibody: D291222(PRKCA (phospho-Thr638) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes