The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]
Applications:
WB, IHC
Name of antibody:
PRKCD (phospho-Ser299)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser299 of human PRKCD
Full name:
protein kinase C delta
Synonyms:
MAY1; PKCD; ALPS3; CVID9; nPKC-delta
SwissProt:
Q05655
IHC positive control:
Human tonsil
IHC Recommend dilution:
50-100
WB Predicted band size:
78 kDa
WB Positive control:
A549, HL-60 lysates
WB Recommended dilution:
500-2000
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Sample Application
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using D291227(PRKCD (phospho-Ser299) Antibody) at dilution 1/50. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: A549, HL-60 lysates, Primary antibody: D291227(PRKCD (phospho-Ser299) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes