The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
Applications:
WB, IHC
Name of antibody:
RB1 (phospho-Thr356)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr356 of human RB1
Full name:
RB transcriptional corepressor 1
Synonyms:
RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1
SwissProt:
P06400
IHC positive control:
Human tonsil
IHC Recommend dilution:
50-100
WB Predicted band size:
106 kDa
WB Positive control:
HL-60, U2OS, C6 lysates
WB Recommended dilution:
500-2000
相关文档
Sample Application
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using D291323(RB1 (phospho-Thr356) Antibody) at dilution 1/50. (Original magnification: ×200)
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-3: HL-60, U2OS, C6 lysates, Primary antibody: D291323(RB1 (phospho-Thr356) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes