Enables promoter-specific chromatin binding activity. Involved in regulation of lipid kinase activity. Acts upstream of or within negative regulation of gene expression. Located in chromosome; cytosol; and nuclear lumen. [provided by Alliance of Genome Resources, Apr 2022]
Applications:
WB, IHC, IF
Name of antibody:
RBL2 (phospho-Ser952)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser952 of human RBL2
Full name:
RB transcriptional corepressor like 2
Synonyms:
Rb2; P130; BRUWAG
SwissProt:
Q08999
IHC positive control:
Human tonsil
IHC Recommend dilution:
50-100
WB Predicted band size:
128 kDa
WB Positive control:
K562, Hela lysates
WB Recommended dilution:
500-2000
IF Positive control:
Hela cells
IF Recommended dilution:
50-200
相关文档
Sample Application
The image is immunofluorescence of Hela cells using D291327(RBL2 (phospho-Ser952) Antibody) at dilution 1/50 (green). Blue: DAPI
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using D291327(RBL2 (phospho-Ser952) Antibody) at dilution 1/50. (Original magnification: ×200)
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-2: K562, Hela lysates, Primary antibody: D291327(RBL2 (phospho-Ser952) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes