This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Applications:
WB
Name of antibody:
RPS6KA1 (phospho-Thr359/Ser363)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of human RPS6KA1
Full name:
ribosomal protein S6 kinase A1
Synonyms:
RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A
SwissProt:
Q15418
WB Predicted band size:
83 kDa
WB Positive control:
3T3, 3T3+TSA, Hela, Hela+TPA, C6 lysates
WB Recommended dilution:
500-2000
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Sample Application
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-5: 3T3, 3T3+TSA, Hela, Hela+TPA, C6 lysates, Primary antibody: D291354(RPS6KA1 (phospho-Thr359/Ser363) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes