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Order NO. D291428
兔抗SMAD3单克隆抗体
英文名 Anti-SMAD3 rabbit monoclonal antibody
别名 SMAD family member 3; LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Rat
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 SMAD3    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D291428-0050 50 ul 预计5日内发货
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D291428-0100 100 ul 预计5日内发货
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属性

Background: The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, May 2022]
Applications: WB, IHC, IF
Name of antibody: SMAD3
Immunogen: Synthetic peptide of human SMAD3
Full name: SMAD family member 3
Synonyms: LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436
SwissProt: P84022
IHC positive control: Human breast cancer
IHC Recommend dilution: 50-100
WB Predicted band size: 48 kDa
WB Positive control: Jurkat, C6, Hela lysates
WB Recommended dilution: 500-2000
IF Positive control: A549 cells
IF Recommended dilution: 50-200
相关文档
Sample Application

  • The image is immunofluorescence of A549 cells using D291428(SMAD3 Antibody) at dilution 1/50 (green). Blue: DAPI

  • The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using D291428(SMAD3 Antibody) at dilution 1/50. (Original magnification: ×200)

  • Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-3: Jurkat, C6, Hela lysates, Primary antibody: D291428(SMAD3 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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