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Order NO. D291456
兔抗SP1单克隆抗体
英文名 Anti-SP1 rabbit monoclonal antibody
别名 Sp1 transcription factor
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 SP1    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D291456-0050 50 ul 预计5日内发货
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D291456-0100 100 ul 预计5日内发货
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属性

Background: The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Applications: WB, IHC, IF
Name of antibody: SP1
Immunogen: Synthetic peptide of human SP1
Full name: Sp1 transcription factor
SwissProt: P08047
IHC positive control: Human colon cancer
IHC Recommend dilution: 50-100
WB Predicted band size: 81 kDa
WB Positive control: K562, 3T3, Hela lysates
WB Recommended dilution: 500-2000
IF Positive control: Hela cells
IF Recommended dilution: 50-200
相关文档
Sample Application

  • The image is immunofluorescence of Hela cells using D291456(SP1 Antibody) at dilution 1/50 (green). Blue: DAPI

  • The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D291456(SP1 Antibody) at dilution 1/50. (Original magnification: ×200)

  • Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-3: K562, 3T3, Hela lysates, Primary antibody: D291456(SP1 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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