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Order NO. D291608
兔抗Ubiquitin K63单克隆抗体
英文名 Anti-Ubiquitin K63 rabbit monoclonal antibody
别名 ubiquitin B; HEL-S-50
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 Ubiquitin K63    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D291608-0050 50 ul 预计5日内发货
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D291608-0100 100 ul 预计5日内发货
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属性

Background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Applications: WB, IHC, IF
Name of antibody: Ubiquitin K63
Immunogen: Synthetic peptide of human Ubiquitin (linkage-specific K63)
Full name: ubiquitin B
Synonyms: HEL-S-50
SwissProt: P0CG47
IHC positive control: Human colon cancer
IHC Recommend dilution: 50-100
WB Predicted band size: 26 kDa
WB Positive control: Hela, A549 lysates
WB Recommended dilution: 500-2000
IF Positive control: Hela cells
IF Recommended dilution: 50-200
相关文档
Sample Application

  • The image is immunofluorescence of Hela cells using D291608(Ubiquitin K63 Antibody) at dilution 1/50 (green). Blue: DAPI

  • The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D291608(Ubiquitin K63 Antibody) at dilution 1/50. (Original magnification: ×200)

  • Gel: 12%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela, A549 lysates, Primary antibody: D291608(Ubiquitin K63 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

  • The image is immunofluorescence of Hela cells using D291608(Ubiquitin K63 Antibody) at dilution 1/50 (green). Blue: DAPI

  • The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D291608(Ubiquitin K63 Antibody) at dilution 1/50. (Original magnification: ×200)

  • Gel: 12%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela, A549 lysates, Primary antibody: D291608(Ubiquitin K63 Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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