This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
Applications:
WB
Name of antibody:
YAP1 (phospho-Ser127)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP1
Full name:
Yes1 associated transcriptional regulator
Synonyms:
YAP; YKI; COB1; YAP2; YAP-1; YAP65
SwissProt:
P46937
WB Predicted band size:
54 kDa
WB Positive control:
Hela, Hela+Calyculin A lysates
WB Recommended dilution:
500-2000
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Sample Application
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela, Hela+Calyculin A lysates, Primary antibody: D291671(YAP1 (phospho-Ser127) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes